Сегодня в продолжении статей «Сколько стоит построить лабораторию» мы поговорим о спектроскопии ядерного магнитного резонанса. ЯМР-спектрометры играют большую роль в работе химика-органика.
ЯМР сегодня не имеет конкурентов в установлении структуры растворимых молекул, позволяет анализировать смеси, и даже дает информацию об их составе. Кроме того, ЯМР применяют для изучения механизмов, кинетики и термодинамики реакций, физико-химических реакций и пр. Большинство ученых считает метод ЯМР одним из самых надежных методов установления структуры и чистоты веществ.
Цена спектрометра изначально определяется его рабочей частотой.
Для рутинных задач оптимально приобретать приборы 400Мгц. Цена – в районе 18-27 миллионов рублей. Кроме того, важно учесть оборудование помещения под ЯМР – около 1 миллиона.
Чтобы сэкономить, можно поискать прибор подешевле, с рук, который уже был в употреблении. В зависимости от состояния цена будет варьироваться в меньшую сторону.
Из дополнительных расходов ещё учитывается то, что в оборудование нужно все время доливать охладители. В среднем, за год работы, ЯМР на 400МГц требует жидкий азот и гелий суммарно тысяч на 300-500 (цена гелия за 1 литр – 1тысяча рублей).
А вот так выглядит 500 МГц прибор, используемый для измерений чуть сложнее рутинных. Справа - магнит, слева - радиоблок.
Теперь о расходных материалах.
Стоимость ЯМР-ампул разнится. Для постоянного использования можно приобретать более бюджетные варианты, цена которых около 100 рублей за штуку. На одного сотрудника нужно иметь примерно 50 штук ампул, либо 50 тысяч рублей.
Также отметим дейтерированные растворители. На один прибор надо 0,5мл растворителя. За год у одного активно работающего химика уходит около 500 образцов, или 250 мл растворителя.
Примерные цены на растворители:
D2O = 2 руб/мл
CDCl3 = 30 руб/мл
ацетон-d6 = 120 руб/мл
ДМСО-d6 = 150 руб/мл
C6D6 = 200 руб/мл
MeCN-d3 = 200 руб/мл
ДМФ-d7 = 2000 руб/мл
Органические вещества редко растворяются в воде, поэтому использовать все время D2O вряд ли выйдет. Большинство умудряются 99% спектров снимать или в CDCl3, или в смесях CCl4 или CS2 с 10% добавкой C6D6 или ацетона. В этом случае в год выходит около 10 тысяч. Однако, в среднем, для нормальной работы с адекватной экономией на одного сотрудника уходит 20-25 тысяч.
А вот как выглядит бенчтоп-ЯМР на 80 МГц. Для понимания масштаба рядом инсулиновый шприц и стакан на 100 мл
В последнее время можно найти много настольных ЯМР-спектрометов на 40-90 МГц. Работают они на постоянных магнитах без сжиженного гелия. Многие химики не работают с такими приборами, считая их «несерьезными», но эти прибор позволяют получать спектры наравне со старыми спектрометрами на 200МГц. Кроме того, такие приборы имеют ещё бонусы – небольшой объем пробы, возможность съемки без дейтерорастворителя, а также возможность вводить образцы простым шприцем, либо подключать оборудование к жидкостному хроматографу.
Стоимость такого прибора в среднем 2-5 миллионов рублей.
Итак, про ЯМР спектрометры сегодня всё. Выбор спектрометра остается делом каждого: выбирать большие приборы на 400Мгц, либо обойтись настольным. Покупать новые приборы или приборы, бывшие в употреблении в хорошем состоянии.
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